The Chromosomes of Some Tasmanian Rodents

نویسنده

  • J. A. DARTNALL
چکیده

All animals were live-trapped in south-east Tasmania. Details of number and sex ar~ shown. in the table. Skins and skulls of all specImens, WIth trapping data, are lodged in the Ta~ma~an Museum, Hobart, and their museum regIstratIOn numbers are also shown in the table. The animals were killed with trichloroethylene and tissues for examination removed immediately after death. The most successful preparations were those from bone marrow. The xlphysternum and about I-I! inches of the sternum were removed and placed in 5 cc of Ringer solution, to w.hich 0.5 mg of demecolcine had been added. The tIssue was minced in this and then allowed to stand at room temperature for an hour. After centrifuging, the deposit was resuspended in warm 0.075 M potassium chloride and the suspension maintained at 37° C for an hour. Fixation was by methanol and acetic acid in a 3: 1 mixture and the preparations were stained in Giemsa.

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تاریخ انتشار 2012